PCR and Electrophoresis Intro
A quick overview of our ideas to help teaching of this topic.
Obtaining cells for PCR
Extracting cells 01:
Cells from cheek cells.
Extracting cells 02:
Cheek cells and saliva sources for DNA sources.
Extracting cells 03:
Centrifuging cells to concentrate samples.
Extracting cells 04:
Centrifuging in ‘sterile conditions’ Cell pellets.
Extracting cells 05:
Obtaining our cheek cell samples.
Extracting cells 06:
Purifying and extracting.
Extracting cells 07:
Using Insta-Gene matrix.
Extracting cells 08:
The heating process for our samples and beads.
Types of pipettes and uses. Fixed and variable volumes.
How to use the fixed and variable volume pipettes.
Loading the small volume samples with your pipette into the appropriate tubes.
PCR preparation 01:
Opening and storing your consumables.
PCR preparation 02:
Using matermix or PCR beads with TAQ polymerase.
PCR preparation 03:
Preparation of samples for PCR tubes; avoiding contamination sources.
PCR preparation 04:
Loading and programming the PCR machine depending upon your particular method.
PCR preparation 05:
Microcentrifuge use to extract DNA samples. Pipetting carefully.
PCR preparation 06:
Mixing primers and mastermix, working to quickly to ensure the technique is effective.
PCR preparation 07:
Using PCR beads. Preparing the sample for PCR.
PCR preparation 08:
Setting up controls. Using the pipetting skills.
PCR preparation 09:
The PCR programming cycle.
PCR preparation 10:
PCR nearing the end of the cycling process.
Making gels for electrophoresis.
Loading dyes and DNA samples added to agarose gels.
Setting up the electrophoresis tank; adding buffer to agarose gels.
Introducing electrophoresis and staining.
Agarose gel results.
Blue stain results.