Genetic Technologies:
- PCR and Electrophoresis Intro
- Obtaining cells for PCR
- Pipetting skills
- PCR preparation
- Electrophoresis
PCR and Electrophoresis Intro
Introduction:
A quick overview of our ideas to help teaching of this topic.
Obtaining cells for PCR
Extracting cells 01:
Cells from cheek cells.
Extracting cells 02:
Cheek cells and saliva sources for DNA sources.
Extracting cells 03:
Centrifuging cells to concentrate samples.
Extracting cells 04:
Centrifuging in ‘sterile conditions’ Cell pellets.
Extracting cells 05:
Obtaining our cheek cell samples.
Extracting cells 06:
Purifying and extracting.
Extracting cells 07:
Using Insta-Gene matrix.
Extracting cells 08:
The heating process for our samples and beads.
Pipetting skills
Pipetting 01:
Types of pipettes and uses. Fixed and variable volumes.
Pipetting 02:
How to use the fixed and variable volume pipettes.
Pipetting 03:
Loading the small volume samples with your pipette into the appropriate tubes.
PCR preparation
PCR preparation 01:
Opening and storing your consumables.
PCR preparation 02:
Using matermix or PCR beads with TAQ polymerase.
PCR preparation 03:
Preparation of samples for PCR tubes; avoiding contamination sources.
PCR preparation 04:
Loading and programming the PCR machine depending upon your particular method.
PCR preparation 05:
Microcentrifuge use to extract DNA samples. Pipetting carefully.
PCR preparation 06:
Mixing primers and mastermix, working to quickly to ensure the technique is effective.
PCR preparation 07:
Using PCR beads. Preparing the sample for PCR.
PCR preparation 08:
Setting up controls. Using the pipetting skills.
PCR preparation 09:
The PCR programming cycle.
PCR preparation 10:
PCR nearing the end of the cycling process.
Electrophoresis
Electrophoresis 01:
Making gels for electrophoresis.
Electrophoresis 02:
Loading dyes and DNA samples added to agarose gels.
Electrophoresis 03:
Setting up the electrophoresis tank; adding buffer to agarose gels.
Electrophoresis 04:
Introducing electrophoresis and staining.
Electrophoresis 05:
Agarose gel results.
Electrophoresis 06:
Flash-gel results.
Electrophoresis 07:
Blue stain results.